Analytical tools for the identification, quantification and valorisation of primary and secondary metabolites
- Date The exact date of the course will be announced later. You will then be contacted to confirm your availability
- Duration 3 days – 15 H Doctoral School equivalent
- Language English/French
- Name of the teacher Mohamed Vall Sidi Boune, Laetitia Shintu, Mehdi Yemloul, Olivier Bornet, Bertrand Legeret, Yonghua Li-Beisson, Magali Roger, Frédéric Carrière
- Administrative head Hélène Launay
- Number of students Group of 5-10 students
- Location IMM + BIP
Description:
The aim is to present and practice different analytical chemistry methods enabling the characterisation of primary or secondary metabolites, and their valorisation. The course will be split between theoretical sessions and practical sessions. In particular, the rational for the choice between different protocols will be compared whether the objective is to perform omics or targeted analysis. Their selectivity for the types of metabolites will be discussed (polar, lipid, polysaccharides, terpenes, etc…). The training will contain:
- Different techniques for extraction.
- Different separation techniques with varying selectivity.
- Different analytical techniques, such as: Thin Layer Chromatography, High Pressure Liquid Chromatography (HPLC) or Gas Chromatography (coupled or not to Mass Spectroscopy), Nuclear Magnetic Resonance (semi-solid (HR-MAS NMR) or liquid) and Fourier Transformed Infra-Red spectroscopies.
- The use of hemi-synthesis strategies for the identification or valorisation of natural product will be presented.
During the practical sessions, the students will identify an unknown metabolite (targeted approach) and will compare the composition of two model mixtures (omics approach) using HPLC, GC, NMR and FT-IR. The students are invited to contact the teachers to bring their own biomass for the extraction and identification of the natural product(s) of their interest.
At the end of the training, the students will be able to extract, partially purify, identify and quantify specific metabolites from their organism of interest (micro- or macroorganisms, from bacteria, yeast, microalgue, plants, insects, etc…). They will be able to adapt the extraction protocol(s) to the biological question (omics/targeted, yield/identification…). They will have knowledge on the complementarity between various analytical methods such as: TLC, GC-MC, HPLC-MS, NMR, FT-IR. They will also be able to design strategies to valorise these natural products by the design of clever hemi-synthesis protocols (as opposed to full synthesis).
Prerequisites
- Basic knowledge on central or secondary metabolic pathways and/or of analytical or organic chemistry.
- Basic knowledge of chromatography (liquid, gas or thin layer chromatographies – HPLC, GC, TLC).
- Basic knowledge of spectroscopies (Mass spectroscopy, Nuclear Magnetic Resonance or infrared spectroscopy).