Screening Platform Marseille-Luminy (PCML)

The screening platform Marseille – Luminy (PCML) is involved in many areas of antiviral research and drug design. Our offers are designed to be as modular as possible, allowing researchers to choose several options (collaborations, services, etc.).
PCML offers its experience and know-how at different levels:
– Screening of inhibitors of viral proteins and enzymes
– Screening on viral enzymes and biochemical characterization
– Screening of inhibitors of protein-protein or protein-ligand interactions
– Miniaturization of biochemical assays in high-throughput
Screening the supply of chemical libraries
From our chemical libraries available at the AFMB
From oriented chemical libraries
Synthesis of oriented chemical libraries and SAR studies
The development of new technologies
– Miniaturisation of tests
– Fluorescence tests
– Test development (type HTRF…)

It is also possible to screen the inhibitory activity of our compounds on an enzyme of interest, or to screen external compounds on our enzymes. For more information: AFBM-Criblage

This collaborative network, labelled IBISA, ChemBioFrance Research Infrastructure and Aix-Marseille Technology Platform, has been in place since 2005 and is composed of PCML, the Marseille-Timone viral screening platform (PCVMT-B.Coutard) and the Interactions, Dynamics and Drug Design platform (INT-3D- X. Morelli). The combination of our respective experiences thus creates a dynamic interface conducive to the development and optimisation of projects.

Facilities
1 chemical library of more than 68,000 compounds
1 type 2 laboratories for radioactivity experiments
1 Beckman I5 robot equipped with a 96-well head and a 384-well head
1 Beckman 4000 robot equipped with a single-well head and an 8-well head
1 MicroBeta scintillation counter (Perkin Elmer)
1 Phospho-imager Amersham Typhoon (Life sciences)
1 PheraStar omega (BMG Labtech)
1 Fluorimeter Tecan Safire21 LISM database

Opening
The service is open to Academics and Industrialists.

Contact

Jean-Claude Guillemot Jean-Claude.Guillemot@univ-amu.fr
Cecilia Eydoux cecilia.eydoux@univ-amu.fr