Proteomic analysis by mass spectrometry LC-MSMS

Mass spectrometry is the main technic used in the Proteomics field and helps in identification of proteins after trypsin digestion, in localization of putative post-translational modifications and in evaluation of relative quantification, from simple to complex protein samples. You will learn principles and practicals of liquid chromatography coupled to electrospray- high resolution mass spectrometry and you will learn how to use bioinformatics tools for proteomic data analysis.

Timeline

1. DAY 1: Morning Principles of mass spectrometry for Proteomics/ Afternoon: if you want , you will perform Trypsin digestion of your own protein samples (before registering the Plinius Program, please contact the IMM facility (proteomique@imm.cnrs.fr) for sample preparation, max 2 per student), and preparation of the mass spectrometer LC-Quadrupole-Orbitrap MS (acquisition program of standard ecoli/Hela digests).
2. Day2: Morning: extraction of Tryptic peptides and analysis of standard digests by proteomics algorithms/afternoon: injection of your own digested samples
3. Day3:  Bioinformatic analysis of your own MS results with specific workflows. Any questions.If no sample, students will train or follow treated samples provided by the Platform.

Students prerequisites

• Knowledge in Biochemistry (sequence & PTM of proteins)

At the end of the workshop, knowledge in proteomics methodologies will help the students to better understand publications describing proteomics and to deeply apply this technology to their own topic research.

Required student equipment

Clean Blouse and personal pipetman of 20µl and 200µl