Microscopy Spring School – IMM

List of trainings

Duration: 5 days – The real date will be precised asap
Doctoral school equivalence: 20 hours Doctoral School equivalent
Language: English but we will adapt the language, depending on the participants
Name of the teachers:
Artemis Kosta, Leon Espinosa , Hugo Le Guenno, Adeline Goulet
Administrative head: Éric Pilet
Number of students: 6 to 12 (first come first served basis).

The Microscopy Spring School will include theoretical and practical courses on microscopy.

The goal of the training is for the students to have an introduction on the local microscopy facilities and techniques, as well as some lectures on more broad subjects as cutting edge structural biology and Alphafold.

Additional information

The basics of microscopy by Leon ESPINOSA (LCB)

French + (+ English slides and comments)
Key words:Le grossissement, la résolution, la profondeur de champ. Schéma du microscope, optique. Les méthodes de contraste en diascopie. L’épifluorescence. Les dispositifs pour l’observations des micro-organismes

Electron Microscopy by Artemis Kosta (IMM)

We will visit the EM facilities (IMM N018), discuss the different techniques available and the questions that can be answered with these instruments. We will prepare and observe negative stained grids. We will plunge freeze grids using the Vitrobot and observe them at Cryo conditions (CryoElectron Microscopy). We will discuss strategies to obtain the answers. Key words: Transmission electron Microscopy, Negative staining, Vitrification, CryoEM

Light Microscopy by Hugo Le Guenno (IMM)

We will gather in the photonics room (IMM N022). Our session will begin with an introduction to the concepts of microscopy. We will then present an example of sample preparation for microbiology. Following this, we will start by capturing images using conventional epifluorescence, followed by confocal microscopy. Through these methods, we will assess the advantages and disadvantages of each. The images generated during this session will be used later in an image analysis training session. Keywords: epifluorescence, confocal, sample preparation

Image Analysis (Hugo Le Guenno, Leon Espinosa)

Introduction to image processing and analysis using the free and open-source software “Fiji”. Through hands-on computer exercises in the computer lab (basement of the PH building), participants will learn to extract quantitative features from their observations. Keywords: image analysis, Fiji