Electron microscopy

List of trainings

Date The exact date of the course will be announced when enough students have registered. You will then be contacted to confirm your availability
Duration 2 days – 12 hours Doctoral School equivalent
Language English
Name of the teachers Eric Durand, Hugo Le Guenno, Artemis Kosta
Administrative head Marie-Line Garron
Number of students Group of 2-6 students

During the course, participants will learn theoretical and practical aspects of sample preparation and data collection for cryo-EM

Lecture

What is CryoEM ? – EM vs structural biology : strategies and objectives.

Practicals

Single particle analysis: quality control by negative staining, vitrification of macromolecules
High Pressure Freezing: vitrification of cells and tissues
Data acquisition: low dose / automatic data collection.
How each machine works: HPF, Glow discharge, Vitrobot.

At the end of the workshop, the student will be able to freeze one grid, to load a sample to the cryo-holder and then to microscope, and to do a small data collection.

Students prerequisites

Registration to the training

Workshop ; January 20-21 2022 (Marseille – ENGLISH )