Proteomics Platform – IMM

The Proteomics Platform of the Institut de Microbiologie de la Méditerranée is part of the Marseille Proteomics Platform (MaP) network, supported by the IMM, La Région, IBiSA, CNRS & AMU; the platform is equipped with a protein sequencer and three mass spectrometers, one of which is a high-resolution spectrometer coupled to a nano-chromatography chain, and another coupled to a proton/deuterium exchange module. The platform is involved in the physico-chemical characterisation of proteins from homogeneous solutions to whole proteomes containing thousands of proteins. It uses Edman sequencing techniques to characterise the primary N-terminal structure, and mass spectrometry to identify proteins by their peptide fingerprint after enzymatic digestion and MS & MS/MS (bottom-up proteomics) analyses. Amino acids targeted by chemical or post-translational modifications (phosphorylation, glutathionylation, acylation, glycosylation, sulphuration, etc.) are also finely characterised. The platform also uses tools to study the conformational dynamics of proteins, by mass spectrometry and H/D exchanges, to shed light on their dynamic or fixed structural regions during protein-protein/protein-ligand interactions or under various physicochemical conditions.

Facilities
Protein Sequencer/Mass Spectrometer: MALDI-ToF (MicroflexII, Bruker)/ESI-Q-ToF(SynaptG1, Waters) coupled with Acquity chromatography (Waters) and HDX/ ESI-Q-Orbitrap module (Q-Exactive plus, Thermofisher) coupled with Ultimate 3000 nanochromatography (Thermofisher).

Opening
The Proteomics Platform of the IMM is open to the constituent units of the IMM and other national EPST laboratories, as well as to private companies. To apply for access, please contact the platform manager.

Contact
Proteomics Platform website
Régine Lebrun proteomique@imm.cnrs.fr