Microscopy Platform

The Microscopy platform of IMM is a service open to the scientific community with two components: Optical Microscopy (OM) and Electron Microscopy (EM). The biological systems analyzed are mainly bacteria, viruses, giant viruses, amoebae, microalgae, but also macromolecules. The methods used are SIM (Structured Illumination Microscopy) super-resolution microscopy, fluorescence microscopy, confocal microscopy, transmission electron microscopy (at room temperature or cryo) and scanning electron microscopy. One of our projects is the development of cryoEM microscopy. The platform is a member of the National Technological Networks (RCCM, SFµ, RTM-FM), participates in local and national training courses and applies a quality approach based on the ISO 9001 standard. The platform is managed by two types of access levels proposed to the users: the use of the equipment with the assistance of the personnel or the disposal of the equipment after training.

Facilities

Electron microscopy equipment:
TEM FEI Tecnai G2 20 TWIN (200KV), Gatan Oneview camera (4Kx4K),
SEM Tescan VEGA3 SBU, Low-Vacuum
High Pressure Freezer Leica HPM100,
Cryo-plunger FEI Vitrobot Mark IV
Cryo-plunger EM CPC Leica
Cryo Holder Gatan 626 et sa station de pompage
Cryo ultramicrotome (UC7), chambre cryo FC7, CEMOVIS, cryosphere, demo camera
Ultramicrotome Leica Ultracut R
Évaporateur de carbone et Glow Discharge Cressington 208c

Optical microscopy equipment and services:
Super-resolution microscopy:
Structured Illumination Microscopy (SIM) DeltaVision OMX SR3D SIM, 2D SIM, 2D SIM-TIRF, Lasers 405, 488, 568, and 640 nm, 3 sCMOS CAMERAS, TIRF, photokinetic illumination, DIC, live-cell environmental control kit
Conventional microscopy (confocal, epifluorescence):
Confocal Olympus FV1000 PicoQuant extensions FLIM et FCS4 continuous lasers (Argon 458/488/515 nm, 2x Helium-Néon 546 et 633 nm et diode 405 nm), 2 pulsed lasers (440 et 470 nm)
Dynamic studies, including interactions between proteins:
FLIM (Fluorescence Life Imaging Microscopy)
FCS (Fluorescence correlation spectroscopy)FLCS Fluorescence Lifetime Correlation Spectroscopy)FCCS (Fluorescence cross-correlation spectroscopy)FRET (Fluorescence Resonance Energy Transfer)

Microscope Axio Vert 200M
Brightfield, phase contrast, DIC, epi-fluorescence

Opening

The service is open to the constituent units of the IMM and other laboratories in the region, as well as to Biotech companies. To apply for access, please contact the platform manager.