High-throughput cloning, expression in bacteria, purification and interaction of recombinant proteins

The IBiSA cloning, expression and protein purification-interaction high-throughput facility can perform all the steps from gene or multi-genes cloning to purified protein samples. This service uses innovative high-throughput strategies and methodologies that have been mostly developed in-house and validated in AFMB using state-of-the-art equipment in partnerships with several structural genomics and big scale projects (More than ten ANR and ten EU grants including; X-TB, MEPNET, SPINE, SPINE II, EMEP, P4EU, VENOMICS, PDZnet, ADDovenom and more).

Since its launch, more than 10.000 proteins have been produced by this facility for customers or collaborators. A large portfolio of automated protocols is available, e.g. a HTP expression E. coli screen at a pace of up to 1152 purifications/analysis per week, a protein-DNA interaction assay (HTP SELEX 96) or a protein-protein interaction assay (HTP Pull-Down or HTP Hold-up in 96/384). New methods can be custom-developed for specific projects. This portfolio of new protocols is accessible once these methods are published.

This service is closely linked to the biophysical and crystallography facilities of the AFMB laboratory.

After having been labelled by IBISA since its creation, PACA-Bioinfo is today one of the regional platforms recognised (and episodically financed) by France-Génomique and the “Institut Français de Bioinformatique”, two national infrastructures resulting from the ” investment in the future ” programme.

Contact

Renaud Vincentelli Renaud.Vincentelli@univ-amu.fr
For more information visit the High-throughput cloning, expression in bacteria, purification and interaction of recombinant proteins platform

Opening

Academics as well as industrials have access to the platform.

Facilities

The HTP platform is equipped with:
– Liquid handling Tecan Freedom EVO 200 with a 96 tips head, an 8-pipette arm and a plate gripper
– Pherastar 96/384/1536 UV-fluorescence reader
– Two Perkin Elmer GX II capillary electrophoresis system for 96/384 sample analysis
– A dedicated minifors culture incubator for bacterial cultures with a maximum capacity of 48 DW24 or 48 DW96 in parallel (Maximum : 1152 to 4608 cultures).
– Two AKTA Xpress chromatography system at 4°C equipped with 8 autonomous modules